Changes in Skeletal Muscle Protein Metabolism Signaling Induced by Glutamine Supplementation and Exercise.

AIM: To evaluate the effects of resistance exercise training (RET) and/or glutamine supplementation (GS) on signaling protein synthesis in adult rat skeletal muscles. METHODS: The following groups were studied: (1) control, no exercise (C); (2) exercise, hypertrophy resistance exercise training protocol (T); (3) no exercise, supplemented with glutamine (G); and (4) exercise and supplemented with glutamine (GT). The rats performed hypertrophic training, climbing a vertical ladder with a height of 1.1 m at an 80° incline relative to the horizontal with extra weights tied to their tails. The RET was performed three days a week for five weeks. Each training session consisted of six ladder climbs. The extra weight load was progressively increased for each animal during each training session. The G groups received daily L-glutamine by gavage (one g per kilogram of body weight per day) for five weeks. The C group received the same volume of water during the same period. The rats were euthanized, and the extensor digitorum longus (EDL) muscles from both hind limbs were removed and immediately weighed. Glutamine and glutamate concentrations were measured, and histological, signaling protein contents, and mRNA expression analyses were performed. RESULTS: Supplementation with free L-glutamine increased the glutamine concentration in the EDL muscle in the C group. The glutamate concentration was augmented in the EDL muscles from T rats. The EDL muscle mass did not change, but a significant rise was reported in the cross-sectional area (CSA) of the fibers in the three experimental groups. The levels of the phosphorylated proteins (pAkt/Akt, pp70S6K/p70S6K, p4E-BP1/4E-BP1, and pS6/S6 ratios) were significantly increased in EDL muscles of G rats, and the activation of p4E-BP1 was present in T rats. The fiber CSAs of the EDL muscles in T, G, and GT rats were increased compared to the C group. These changes were accompanied by a reduction in the 26 proteasome activity of EDL muscles from T rats. CONCLUSION: Five weeks of GS and/or RET induced muscle hypertrophy, as indicated by the increased CSAs of the EDL muscle fibers. The increase in CSA was mediated via the upregulated phosphorylation of Akt, 4E-BP1, p70S6k, and S6 in G animals and 4E-BP1 in T animals. In the EDL muscles from T animals, a decrease in proteasome activity, favoring a further increase in the CSA of the muscle fibers, was reported.

Effects of high EPA and high DHA fish oils on changes in signaling associated with protein metabolism induced by hindlimb suspension in rats.

The effects of either eicosapentaenoic (EPA)- or docosahexaenoic (DHA)-rich fish oils on hindlimb suspension (HS)-induced muscle disuse atrophy were compared. Daily oral supplementations (0.3 mL/100 g b.w.) with mineral oil (MO) or high EPA or high DHA fish oils were performed in adult rats. After 2 weeks, the animals were subjected to HS for further 2 weeks. The treatments were maintained alongside HS At the end of 4 weeks, we evaluated: body weight gain, muscle mass and fat depots, composition of fatty acids, cross-sectional areas (CSA) of the soleus muscle and soleus muscle fibers, activities of cathepsin L and 26S proteasome, and content of carbonylated proteins in the soleus muscle. Signaling pathway activities associated with protein synthesis (Akt, p70S6K, S6, 4EBP1, and GSK3-beta) and protein degradation (atrogin-1/MAFbx, and MuRF1) were evaluated. HS decreased muscle mass, CSA of soleus muscle and soleus muscle fibers, and altered signaling associated with protein synthesis (decreased) and protein degradation (increased). The treatment with either fish oil decreased the ratio of omega-6/omega-3 fatty acids and changed protein synthesis-associated signaling. EPA-rich fish oil attenuated the changes induced by HS on 26S proteasome activity, CSA of soleus muscle fibers, and levels of p-Akt, total p70S6K, p-p70S6K/total p70S6K, p-4EBP1, p-GSK3-beta, p-ERK2, and total ERK 1/2 proteins. DHA-rich fish oil attenuated the changes induced by HS on p-4EBP1 and total ERK1 levels. The effects of EPA-rich fish oil on protein synthesis signaling were more pronounced. Both EPA- and DHA-rich fish oils did not impact skeletal muscle mass loss induced by non-inflammatory HS.

Apresentação do sistema CSS (Compliance Science System) como meio de avaliação de uso dos aparelhos de ancoragem extrabucal / Presentation of the CSS System (Compliance Science System) as a way to evaluate the use of headgear anchorage appliances

O objetivo deste artigo é apresentarmos um sistema de avaliação do uso de aparelhos extrabucais. Este sistema, chamado Compliance Science System (CSS) constitui-se de um módulo eletrônico instalado na tala do aparelho extrabucal, de um leitor deste módulo que é conectado ao computador e de um programa específico para leitura dos registros feitos pelo módulo. O sistema CSS tem a finalidade de quantificar o tempo de uso do aparelho extrabucal, determinando o nível de cooperação do paciente durante os períodos do dia em que foi utilizado. Com a finalidade de ilustrar a utilização do sistema CSS, apresentamos dois (2) casos clínicos de pacientes portadores de maloclusão de Classe II de Angle esquelética com overjet acentuado e que foram tratados com a utilização de aparelho extrabucal de puxada cervical do tipo KLoehn

A informática no consultório de ortodontia: diretrizes / Computers in an orthodontic office: directives

A ortodontia vem tentando acompanhar o desenvolvimento acelerado da informática. Pela quantidade de informações, já não é fácil acompanhar a evolução científica e tecnológica da ortodontia e ultimamente a informática quase que impõe a sua presença e uso. Apesar de serem profissionais já acostumados a lidar com máquinas desde a sua formação, tais como o compressor, a alta rotação, equipo, aparelhos de raio X, typodont, máquina de soldar, aparelhos de esterilização e outros, os ortodontistas apresentam diversos graus de dificuldade ao lidarem com computadores e seus programas. Este artigo sugere uma maneira básica e abrangente sobre o que saber e fazer para a utilização de máquinas e programas oferecidos pela informática, para a estruturação de um consultório de ortodontia, abordando aspectos de gerenciamento e administração, diagnóstico cefalométrico, gerenciamento de imagens, comunicação e Internet